Posted by
Straatman, Kees (Dr.) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Olympus-ZDC-2-vs-Nikon-PFS-tp6370076p6558211.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
I agree with aryeh concerning PFS and can add that the ZDC also works on multiwall plates using LWD objectives.
Kees
Dr Ir K.R. Straatman
Senior Experimental Officer
Centre for Core Biotechnology Services
College of Medicine, Biological Sciences and Psychology
http://www.le.ac.uk/biochem/microscopy/home.html-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Aryeh Weiss
Sent: 07 July 2011 13:22
To:
[hidden email]
Subject: Re: Focus mechanisms... Re: Olympus ZDC 2 vs. Nikon PFS
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
On 7/7/11 2:51 PM, Mario Faretta wrote:
> useful and efficient. We tested it on different magnifications and
> numerical apertures with good results. The available objectives working
> with the PFS cover a wide range of nees, maybe the only limitation is
> that they usually have short working distance, but I am not up to date
> on the list of available solutions, maybe better for you to check if
> this is a strong limitation.
I can add that we used the PFS with LWD air objectives and plastic
multiwell plates, and it works well. However, if you have been using it
with high NA short WD objectives, you will need to reset the offset to
get it to lock.
It is important that the plate not move too far out of focus when moving
between wells. However, this was only a problem for us when we had a bad
holder that seriously deformed the multiwell plate.
--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel
Ph: 972-3-5317638
FAX: 972-3-7384051