Posted by
Watkins, Simon C on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Olympus-ZDC-2-vs-Nikon-PFS-tp6370076p6558405.html
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With all these devices you need to make sure that the blocking glass in the system does not confound your experiments. for example if you work in the near IR (Cy5-7) or near UV (Fura) you should check the light transmission for the chosen device to ensure compatibility.
S
Simon C. Watkins Ph.D, FRC Path
Professor and Vice Chair Cell Biology and Physiology
Professor Immunology Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St
Pittsburgh PA 15261
412-352-2277
www.cbi.pitt.edu
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Straatman, Kees R. (Dr.)
Sent: Thursday, July 07, 2011 8:38 AM
To:
[hidden email]
Subject: Re: Focus mechanisms... Re: Olympus ZDC 2 vs. Nikon PFS
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I agree with aryeh concerning PFS and can add that the ZDC also works on multiwall plates using LWD objectives.
Kees
Dr Ir K.R. Straatman
Senior Experimental Officer
Centre for Core Biotechnology Services
College of Medicine, Biological Sciences and Psychology
http://www.le.ac.uk/biochem/microscopy/home.html-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Aryeh Weiss
Sent: 07 July 2011 13:22
To:
[hidden email]
Subject: Re: Focus mechanisms... Re: Olympus ZDC 2 vs. Nikon PFS
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On 7/7/11 2:51 PM, Mario Faretta wrote:
> useful and efficient. We tested it on different magnifications and
> numerical apertures with good results. The available objectives working
> with the PFS cover a wide range of nees, maybe the only limitation is
> that they usually have short working distance, but I am not up to date
> on the list of available solutions, maybe better for you to check if
> this is a strong limitation.
I can add that we used the PFS with LWD air objectives and plastic
multiwell plates, and it works well. However, if you have been using it
with high NA short WD objectives, you will need to reset the offset to
get it to lock.
It is important that the plate not move too far out of focus when moving
between wells. However, this was only a problem for us when we had a bad
holder that seriously deformed the multiwell plate.
--aryeh
--
Aryeh Weiss
School of Engineering
Bar Ilan University
Ramat Gan 52900 Israel
Ph: 972-3-5317638
FAX: 972-3-7384051