Re: Pulse compression and in vivo imaging

Posted by Philippe clemenceau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Pulse-compression-and-in-vivo-imaging-tp6557894p6562609.html

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Hello Stephane,

My company, Imagine Optic, manufactures high performance Adaptive Optics
loops and we have lots of experience and very good results in microscopy,
especially non-linear microscopy and super resolution.

You can go to
http://www.imagine-optic.com/iop_applications_adaptive-optics_microscopy-lif
e-sciences_en.php to see some images.

To avoid too commercial language on the list, I will send you a private
email.

Kind regards,

Philippe Clémenceau, Division Manager

Imagine Optic Inc./Axiom Optics
Ph:[phone]
Cell: + 1 [phone]
1 Broadway, 14th floor
Fax: [phone]
Cambridge,  MA 02142
www.imagine-optic.com

Scientific Imaging , Laser Characterization & Laser based NDT

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Stéphane Pagès
Sent: Friday, July 08, 2011 4:23 AM
To: [hidden email]
Subject: Re: Pulse compression and in vivo imaging

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Thanks Craig,
Are you aware of some commercial optical adaptive systems  easily
implementable on a 2-P microscope ?
or is the technology still in development in labs ?



2011/7/7 Craig Brideau <[hidden email]>

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> To join, leave or search the confocal microscopy listserv, go to:
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> *****
>
> If scattering is the issue then adaptive optics will be more advantageous
> than dispersion control.  The adaptive optics will help compensate
somewhat

> for the scattering and aberrations induced by the tissue.  To get good 2P
> imaging you need a good focal spot more-so than you need a perfectly
> transform limited pulse.  Adaptive optics will help keep your focus
> together
> as you try to image deeply.  That said, dispersion compensation will help
> somewhat so if you already have the necessary equipment then try it.
>
> Craig
>
>
>
> On Thu, Jul 7, 2011 at 4:44 AM, Stéphane Pagès <
> [hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi everybody,
> > I am planning to image fluorescent neurons in vivo approximately  200 um
> > below the pia with a standard Ti:Sa laser.
> > I wonder if there is a clear advantage to use pulse compression to
> > compensate for dispersion of pulses due to tissue.
> > I understand theoretical arguments in favor of pulse compression.
> > However from an experimental point of view, are there some people here

in
> > the list that have experienced some gain (in lowering the intensity of
> the
> > exciting beam for example).
> > Any comments would be greatly appreciated.
> > Thanks a lot
> > Stephane
> >
>

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