Re: Pulse compression and in vivo imaging

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Pulse-compression-and-in-vivo-imaging-tp6557894p6563178.html

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Heh.  That was quick for the commercial response. @:-) Some of the adaptive
optics companies, like these guys, have drop-in systems.  I haven't
personally used one but from what I've seen and heard they are relatively
easy to add to existing setups.  It is interesting how quickly the
commercial implementation of AO has progressed. It has gone from lab-only to
fast add-on within a couple years.  I guess everyone is hoping that adaptive
optics can do for microscopy what it has done for astronomy.

Craig


On Fri, Jul 8, 2011 at 8:08 AM, Philippe Clemenceau <
[hidden email]> wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
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> *****
>
> Hello Stephane,
>
> My company, Imagine Optic, manufactures high performance Adaptive Optics
> loops and we have lots of experience and very good results in microscopy,
> especially non-linear microscopy and super resolution.
>
> You can go to
>
> http://www.imagine-optic.com/iop_applications_adaptive-optics_microscopy-lif
> e-sciences_en.php to see some images.
>
> To avoid too commercial language on the list, I will send you a private
> email.
>
> Kind regards,
>
> Philippe Clémenceau, Division Manager
>
> Imagine Optic Inc./Axiom Optics
> Ph:[phone]
> Cell: + 1 [phone]
> 1 Broadway, 14th floor
> Fax: [phone]
> Cambridge,  MA 02142
> www.imagine-optic.com
>
> Scientific Imaging , Laser Characterization & Laser based NDT
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On
> Behalf Of Stéphane Pagès
> Sent: Friday, July 08, 2011 4:23 AM
> To: [hidden email]
> Subject: Re: Pulse compression and in vivo imaging
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Thanks Craig,
> Are you aware of some commercial optical adaptive systems  easily
> implementable on a 2-P microscope ?
> or is the technology still in development in labs ?
>
>
>
> 2011/7/7 Craig Brideau <[hidden email]>
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > If scattering is the issue then adaptive optics will be more advantageous
> > than dispersion control.  The adaptive optics will help compensate
> somewhat
> > for the scattering and aberrations induced by the tissue.  To get good 2P
> > imaging you need a good focal spot more-so than you need a perfectly
> > transform limited pulse.  Adaptive optics will help keep your focus
> > together
> > as you try to image deeply.  That said, dispersion compensation will help
> > somewhat so if you already have the necessary equipment then try it.
> >
> > Craig
> >
> >
> >
> > On Thu, Jul 7, 2011 at 4:44 AM, Stéphane Pagès <
> > [hidden email]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > Hi everybody,
> > > I am planning to image fluorescent neurons in vivo approximately  200
> um
> > > below the pia with a standard Ti:Sa laser.
> > > I wonder if there is a clear advantage to use pulse compression to
> > > compensate for dispersion of pulses due to tissue.
> > > I understand theoretical arguments in favor of pulse compression.
> > > However from an experimental point of view, are there some people here
> in
> > > the list that have experienced some gain (in lowering the intensity of
> > the
> > > exciting beam for example).
> > > Any comments would be greatly appreciated.
> > > Thanks a lot
> > > Stephane
> > >
> >
>
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