Posted by
Stanislav Vitha on
Jul 08, 2011; 7:57pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Pulse-compression-and-in-vivo-imaging-tp6557894p6563930.html
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Also, it depends on the pulse width.
the shorter the pulse, the more you may need the dispersion control as you go
deeper in the sample.
On our system with 10 fs pulses, we really cannot live without pre-chirp
(dispersion control). Your standard oscillator (~100-fs pulses?) is much more
forgiving.
Stan Vitha
Microscopy and Imaging Center
Texas A&M University
On Thu, 7 Jul 2011 13:20:02 -0600, Craig Brideau
<
[hidden email]> wrote:
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>If scattering is the issue then adaptive optics will be more advantageous
>than dispersion control. The adaptive optics will help compensate somewhat
>for the scattering and aberrations induced by the tissue. To get good 2P
>imaging you need a good focal spot more-so than you need a perfectly
>transform limited pulse. Adaptive optics will help keep your focus together
>as you try to image deeply. That said, dispersion compensation will help
>somewhat so if you already have the necessary equipment then try it.
>
>Craig
>
>
>
>On Thu, Jul 7, 2011 at 4:44 AM, Stéphane Pagès <
>
[hidden email]> wrote:
>
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>> Hi everybody,
>> I am planning to image fluorescent neurons in vivo approximately 200 um
>> below the pia with a standard Ti:Sa laser.
>> I wonder if there is a clear advantage to use pulse compression to
>> compensate for dispersion of pulses due to tissue.
>> I understand theoretical arguments in favor of pulse compression.
>> However from an experimental point of view, are there some people here
in
>> the list that have experienced some gain (in lowering the intensity of the
>> exciting beam for example).
>> Any comments would be greatly appreciated.
>> Thanks a lot
>> Stephane
>>