Posted by
Stanislav Vitha on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Pulse-compression-and-in-vivo-imaging-tp6557894p6566316.html
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Craig,
We use a pair of chirp mirrors, typically set-up with 7 or 8 pairs of bounces
(depends on the objective used). We slightly over-compensate and have
several mm of BK7 glass in the laser path, that we can take out as needed for
deep imaging.
The slight downside of the current set up is that changing the number of
bounces on the chirp mirrors requires laser re-alignment.
Stan
On Fri, 8 Jul 2011 14:32:40 -0600, Craig Brideau <
[hidden email]>
wrote:
>*****
>
>Actually the Chameleon was designed for 140fs pulses for this reason. For a
>'typical' microscope system, transform-limited 140 fs gives you minimum
>dispersion. On the other hand, if you have a pulse compressor you want to
>start with as short a pulse as possible as the compressor can keep
>dispersion in check for you.
>If I might ask, Stan, what do you use for a compressor on your 10fs laser?
>
>Craig
>
>
>On Fri, Jul 8, 2011 at 1:57 PM, Stanislav Vitha <
[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
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>>
>> Also, it depends on the pulse width.
>> the shorter the pulse, the more you may need the dispersion control as you
>> go
>> deeper in the sample.
>> On our system with 10 fs pulses, we really cannot live without pre-chirp
>> (dispersion control). Your standard oscillator (~100-fs pulses?) is much
>> more
>> forgiving.
>>
>> Stan Vitha
>> Microscopy and Imaging Center
>> Texas A&M University
>>