Posted by
Cameron Nowell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Olympus-ZDC-2-vs-Nikon-PFS-tp6370076p6588383.html
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Howdy,
We use the MetaMorph autofocus quite often. If you tweak it right (binning, range fr searching etc.) it can be quite fast. Also the advantage of any image based atofocus system is that it will compensate for your sample moving up or down, or flattening out, over time. Systems like the Olympus ZDC (which we use on our confocal system), Nikon PF etc all find the coverslip again. If you sample doesn't move in the same way as the cover slip you may have issues with the focus drifting anyway.
Of the three laser based focus systems i think Nions is the fastests, Olypus the slowest and Leica in between. All can be a bit of a pain to set up though.
Cheers
Cam
Cameron J. Nowell
Microscpy Manager
Central Resource for Advanced Microscopy
Ludwig Insttue for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
Office: +61 3 9341 3155
Mobile: +61422882700
Fax: +61 3 9341 3104
http://www.ludwig.edu.au/branch/research/platform/microscopy.htm
________________________________
From: Confocal Microscopy List on behalf of Axel Kurt Preuss
Sent: Fri 7/8/2011 8:58 PM
To:
[hidden email]
Subject: Re: Olympus ZDC 2 vs. Nikon PFS
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Dear Gareth
The Metamorph software AF is OK but not perfect crisp. It helps if you want to image thicker plastic plates. Leica continuous focus needs glass or very thin plastic well bottoms.
Leica s continuous focus is much faster than Olympus. I haven't looked at Nikon s PFS but my users normally tend to switch it off. Olympus AF needs also to be within a relatively narrow range
Hope that helps
Axel
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Gareth Howell
Sent: Friday, 8 July, 2011 6:26 PM
To:
[hidden email]
Subject: Re: Olympus ZDC 2 vs. Nikon PFS
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Hi, on a related note: We are currently planning to put together a live cell imaging station controlled through Metamorph software. We were thinking of utilising the MM autofocus capability (of which I have no experience) so I was wondering what you guys thought of it compared to say splashing out a little more and getting something along the lines of PFS and ZDC on a new stand?
Thanks
Gareth
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