Settings adjustment with depth for a Zeiss LSM 510

Posted by Pedro Almada on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Settings-adjustment-with-depth-for-a-Zeiss-LSM-510-tp6622398.html

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Dear listers,

Does anyone know of a VBA macro for the Zeiss Meta which allows users to
adjust settings such as gain and laser power according to current focus for
the Zeiss LSM 510? I am interested in developing this myself but if there is
something already made, that would be great...

If you are curious what it's for, it's for some of our users who image whole
drosophila brains for structural information. Their common complaint is that
half-way through their z-stack they loose image brightness until the last
slices are barely visible. Clearing methods like Methyl salicilate aren't
really adequate for neuronal structure I'm told, which leads me to consider
this method.

Either way, any information is welcome.

Thanks for reading,
Cheers,
Pedro Almada
*
*
Research and Microscopy Technician
Unidade de Imagiologia Celular,
*Instituto Gulbenkian de Ciência*
Rua da Quinta Grande, 6
2780-156 Oeiras

Phone: + 351 214 464 607
Ext: 607