Re: Settings adjustment with depth for a Zeiss LSM 510

Posted by G. Esteban Fernandez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Settings-adjustment-with-depth-for-a-Zeiss-LSM-510-tp6622398p6622672.html

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The acquisition software (LSM or ZEN) has that function built in, no need
for a macro.  It is called something like "Z brightness correction" and is
in the Z stack panel.

Software manuals for LSM and ZEN can be found at the Zeiss FTP site here:
ftp://lsm.zeiss.com/LSM/User_Area/Manuals/

-Esteban

On Tue, Jul 26, 2011 at 7:26 AM, Pedro Almada <[hidden email]> wrote:

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> Dear listers,
>
> Does anyone know of a VBA macro for the Zeiss Meta which allows users to
> adjust settings such as gain and laser power according to current focus for
> the Zeiss LSM 510? I am interested in developing this myself but if there
> is
> something already made, that would be great...
>
> If you are curious what it's for, it's for some of our users who image
> whole
> drosophila brains for structural information. Their common complaint is
> that
> half-way through their z-stack they loose image brightness until the last
> slices are barely visible. Clearing methods like Methyl salicilate aren't
> really adequate for neuronal structure I'm told, which leads me to consider
> this method.
>
> Either way, any information is welcome.
>
> Thanks for reading,
> Cheers,
> Pedro Almada
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> Unidade de Imagiologia Celular,
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