Posted by
Michael Schell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Incubator-box-heating-mystery-tp6627357p6627372.html
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Esteban,
We use the same box as you do, mounted on an Axiovert 200M. If you open the doors and cause the temperature to drop, then close the doors again, the temperature overshoots to about 39 degrees. Then it gradually cools back to 37. Once it reaches 37, it will remain that way for a week if you don't open the doors. I attribute the overshoot to temperature gradients inside the box, and I suspect that placement of the sensor matters. Have you tried moving the sensor to different locations i the box, and is the sensor placed differently on the two kinds of microscope you use?
Michael
Michael J. Schell, Ph.D., CIV, USUHS
Assist. Professor
Dept. of Pharmacology
Uniformed Services University
4301 Jones Bridge Rd.
Bethesda, MD 20814-3220
tel: (301) 295-3249
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>>> "G. Esteban Fernandez" 07/27/11 3:02 PM >>>
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Hello everyone,
I have a very strage problem with an incubator box heating up. I have two
live cell imaging microscopes enclosed in PeCon incubator boxes (I think
they are Incubator XL). The microscopes are in two separate buildings. One
microscope heats up significantly past the set temp. (above 41 degrees, set
to 37 degrees) even though the temp. is being measured and reported
correctly to the electronics (confirmed by a glass thermometer). The other
microscope works perfectly and holds at 37 degrees for days. The strange
thing is that when I put the temp. control components that work well
(heater, control electronics, temp. sensor, and all cables) onto the
microscope that heats up, it still heats up, even though the components work
perfectly on the other microscope! Any ideas why this might be happening,
why the temp. control equipment works on one microscope but not on the
other?
The temp. equipment is stand-alone, not connected to a computer or to the
microscope in any way. One microscope is a Zeiss Axiovert 200M (the one
that works) and the other is a Leica DM IRE2 (heats up) in separate
buildings; the incubator boxes surrounding them are very similar. Any ideas
on this mystery are welcome.
Thanks,
Esteban
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