Posted by
kevin-23 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Incubator-box-heating-mystery-tp6627357p6627431.html
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I would check the location of the temperature sensor to make sure that it is as close as possible to the stage on the transmitted light arm.
I hope that this helps.
Kevin L. Ryan
Leica Microscopes and Imaging
W. Nuhsbaum Inc.
Shaker Heights, Ohio
1-800-368-3368
Cell: 216-401-4079
[hidden email]
www.nuhsbaum.com
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of G. Esteban Fernandez
Sent: Wednesday, July 27, 2011 3:02 PM
To:
[hidden email]
Subject: Incubator box heating mystery
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Hello everyone,
I have a very strage problem with an incubator box heating up. I have two
live cell imaging microscopes enclosed in PeCon incubator boxes (I think
they are Incubator XL). The microscopes are in two separate buildings. One
microscope heats up significantly past the set temp. (above 41 degrees, set
to 37 degrees) even though the temp. is being measured and reported
correctly to the electronics (confirmed by a glass thermometer). The other
microscope works perfectly and holds at 37 degrees for days. The strange
thing is that when I put the temp. control components that work well
(heater, control electronics, temp. sensor, and all cables) onto the
microscope that heats up, it still heats up, even though the components work
perfectly on the other microscope! Any ideas why this might be happening,
why the temp. control equipment works on one microscope but not on the
other?
The temp. equipment is stand-alone, not connected to a computer or to the
microscope in any way. One microscope is a Zeiss Axiovert 200M (the one
that works) and the other is a Leica DM IRE2 (heats up) in separate
buildings; the incubator boxes surrounding them are very similar. Any ideas
on this mystery are welcome.
Thanks,
Esteban