Re: Settings adjustment with depth for a Zeiss LSM 510

Posted by Jacqueline Ross on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Settings-adjustment-with-depth-for-a-Zeiss-LSM-510-tp6622398p6628345.html

Hi Pedro,

We also have an LSM 510 META. If you have the Guided Tour PDF, page 45 explains how to use the Auto Z Brightness correction. However, you can only set 2 positions (with associated gain/offset) so sometimes this isn't enough.

Kind regards,

Jacqui

Jacqueline Ross

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Pedro Almada
Sent: Wednesday, 27 July 2011 10:25 p.m.
To: [hidden email]
Subject: Re: Settings adjustment with depth for a Zeiss LSM 510

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Hi Esteban and Martin,

Thanks alot for the help. I admit I wasn't aware of the option being in the
software itself. I'll try and have a look at their preparations myself and
check for oversaturation (if I remember their pictures correctly, that
sounds like a definite possibility).

Thanks again, this is a very helpful and interesting list.

All the best,
Pedro

On 26 July 2011 16:37, G. Esteban Fernandez
<[hidden email]>wrote:

> The acquisition software (LSM or ZEN) has that function built in, no need
> for a macro.  It is called something like "Z brightness correction" and is
> in the Z stack panel.
>
> Software manuals for LSM and ZEN can be found at the Zeiss FTP site here:
> ftp://lsm.zeiss.com/LSM/User_Area/Manuals/
>
> -Esteban
>
> On Tue, Jul 26, 2011 at 7:26 AM, Pedro Almada <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear listers,
>>
>> Does anyone know of a VBA macro for the Zeiss Meta which allows users to
>> adjust settings such as gain and laser power according to current focus
>> for
>> the Zeiss LSM 510? I am interested in developing this myself but if there
>> is
>> something already made, that would be great...
>>
>> If you are curious what it's for, it's for some of our users who image
>> whole
>> drosophila brains for structural information. Their common complaint is
>> that
>> half-way through their z-stack they loose image brightness until the last
>> slices are barely visible. Clearing methods like Methyl salicilate aren't
>> really adequate for neuronal structure I'm told, which leads me to
>> consider
>> this method.
>>
>> Either way, any information is welcome.
>>
>> Thanks for reading,
>> Cheers,
>> Pedro Almada
>> *
>> *
>> Research and Microscopy Technician
>> Unidade de Imagiologia Celular,
>> *Instituto Gulbenkian de Ciência*
>> Rua da Quinta Grande, 6
>> 2780-156 Oeiras
>>
>> Phone: + 351 214 464 607
>> Ext: 607
>>
>