Re: Settings adjustment with depth for a Zeiss LSM 510

Posted by G. Esteban Fernandez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Settings-adjustment-with-depth-for-a-Zeiss-LSM-510-tp6622398p6628678.html

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In case it's useful enough to you to warrant an upgrade, the new Zeiss
confocal software, ZEN, allows more than two set points plus variable laser
power in addition to variable detector settings.  (No commercial interest)

-Esteban




On Wed, Jul 27, 2011 at 5:55 PM, Jacqui Ross <[hidden email]>wrote:

> Hi Pedro,
>
> We also have an LSM 510 META. If you have the Guided Tour PDF, page 45
> explains how to use the Auto Z Brightness correction. However, you can only
> set 2 positions (with associated gain/offset) so sometimes this isn't
> enough.
>
> Kind regards,
>
> Jacqui
>
> Jacqueline Ross
>
> Biomedical Imaging Microscopist
> Biomedical Imaging Research Unit
> School of Medical Sciences
> Faculty of Medical & Health Sciences
> The University of Auckland
> Private Bag 92019
> Auckland, NEW ZEALAND
>
> Tel: [phone] Ext 87438
> Fax: [phone]
>
> http://www.fmhs.auckland.ac.nz/sms/biru/
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Pedro Almada
> Sent: Wednesday, 27 July 2011 10:25 p.m.
> To: [hidden email]
> Subject: Re: Settings adjustment with depth for a Zeiss LSM 510
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Hi Esteban and Martin,
>
> Thanks alot for the help. I admit I wasn't aware of the option being in the
> software itself. I'll try and have a look at their preparations myself and
> check for oversaturation (if I remember their pictures correctly, that
> sounds like a definite possibility).
>
> Thanks again, this is a very helpful and interesting list.
>
> All the best,
> Pedro
>
> On 26 July 2011 16:37, G. Esteban Fernandez
> <[hidden email]>wrote:
>
> > The acquisition software (LSM or ZEN) has that function built in, no need
> > for a macro.  It is called something like "Z brightness correction" and
> is
> > in the Z stack panel.
> >
> > Software manuals for LSM and ZEN can be found at the Zeiss FTP site here:
> > ftp://lsm.zeiss.com/LSM/User_Area/Manuals/
> >
> > -Esteban
> >
> > On Tue, Jul 26, 2011 at 7:26 AM, Pedro Almada <[hidden email]> wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> *****
> >>
> >> Dear listers,
> >>
> >> Does anyone know of a VBA macro for the Zeiss Meta which allows users to
> >> adjust settings such as gain and laser power according to current focus
> >> for
> >> the Zeiss LSM 510? I am interested in developing this myself but if
> there
> >> is
> >> something already made, that would be great...
> >>
> >> If you are curious what it's for, it's for some of our users who image
> >> whole
> >> drosophila brains for structural information. Their common complaint is
> >> that
> >> half-way through their z-stack they loose image brightness until the
> last
> >> slices are barely visible. Clearing methods like Methyl salicilate
> aren't
> >> really adequate for neuronal structure I'm told, which leads me to
> >> consider
> >> this method.
> >>
> >> Either way, any information is welcome.
> >>
> >> Thanks for reading,
> >> Cheers,
> >> Pedro Almada
> >> *
> >> *
> >> Research and Microscopy Technician
> >> Unidade de Imagiologia Celular,
> >> *Instituto Gulbenkian de Ciência*
> >> Rua da Quinta Grande, 6
> >> 2780-156 Oeiras
> >>
> >> Phone: + 351 214 464 607
> >> Ext: 607
> >>
> >
>