http://confocal-microscopy-list.275.s1.nabble.com/Incubator-box-heating-mystery-tp6627357p6631915.html
(particularly over the weekends in our case). I'd be interested though
in both temperature and humidity. We are constantly battling with both
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> We had quite a bit of trouble with our room ventilation until quite
> recently. We purchased an inexpensive USB datalogger to continuously record
> temperature and humidity in the room. It picked up large swings in
> temperature and humidity that the ventilation system was supposed to
> prevent. The data we collected finally convinced the building contractor to
> install proper humidity controls and hardware for our room. I recommend
> that every facility should keep logs of temperature and humidity. The
> loggers are quite cheap now, and can be programmed, detached from the
> computer, and left unattended for months. We would move ours around the
> room to get a feel for any gradients, heat loads from equipment, etc.
>
> Craig
>
> On Thu, Jul 28, 2011 at 11:03 AM, Zac Arrac Atelaz <
[hidden email]>wrote:
>
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>>
>> Esteban:
>>
>> Your problem might be the changing position of the termometer of the
>> system, one interesting trial will be putting the overheating system in the
>> non overheating system, to see if is that part fails or remain working as
>> the trial you make the way around.
>>
>> If your system is failing you should consider one incubator as the one we
>> have, this is not the huge box making the microscope unreachable, it is the
>> size of the insert in the stage and it has 4 points heating your sample, the
>> cover, the objective used, and the water heater, we have never had a
>> temperature overshoot as the mentioned by Michael, even if we open doors, or
>> change room temperature ( recorded from 18 to 27°C) the shift in temperature
>> observed trough time in the incubator is about 0.5°C over a 25h period. In
>> such long experiments we have people reviewing samples as you dont want to
>> let the confocal working if the sample has suffered unwanted changes, by the
>> way the brand of the one we have is a INU - Tokai hit incubator.
>>
>> I hope this helps a little.
>>
>> Gabriel Orozco Hoyuela
>>
>>
>> > Date: Wed, 27 Jul 2011 12:01:32 -0700
>> > From:
[hidden email]
>> > Subject: Incubator box heating mystery
>> > To:
[hidden email]
>> >
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>> >
>> > Hello everyone,
>> >
>> > I have a very strage problem with an incubator box heating up. I have two
>> > live cell imaging microscopes enclosed in PeCon incubator boxes (I think
>> > they are Incubator XL). The microscopes are in two separate buildings.
>> One
>> > microscope heats up significantly past the set temp. (above 41 degrees,
>> set
>> > to 37 degrees) even though the temp. is being measured and reported
>> > correctly to the electronics (confirmed by a glass thermometer). The
>> other
>> > microscope works perfectly and holds at 37 degrees for days. The strange
>> > thing is that when I put the temp. control components that work well
>> > (heater, control electronics, temp. sensor, and all cables) onto the
>> > microscope that heats up, it still heats up, even though the components
>> work
>> > perfectly on the other microscope! Any ideas why this might be happening,
>> > why the temp. control equipment works on one microscope but not on the
>> > other?
>> >
>> > The temp. equipment is stand-alone, not connected to a computer or to the
>> > microscope in any way. One microscope is a Zeiss Axiovert 200M (the one
>> > that works) and the other is a Leica DM IRE2 (heats up) in separate
>> > buildings; the incubator boxes surrounding them are very similar. Any
>> ideas
>> > on this mystery are welcome.
>> >
>> > Thanks,
>> > Esteban
>>
>>
>