http://confocal-microscopy-list.275.s1.nabble.com/Incubator-box-heating-mystery-tp6627357p6634448.html
between 35 and 20%. Our humidity only drops below 20% in the winter, and
our metal optical tables seem to keep it under control anyway. Your mileage
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
>
> Adding to Craig explanation, and something I remember after his explanation
> is that the specs for optical microscopes are mainly evaluated in a small
> range between 220 and 628 meters above sea level, so if you take the system
> to a higher altitude the heat dispersion might be slower than originally
> calculated for the system, this is obviated some times, as the effect is not
> too big but this might have some effect on your results.
>
>
http://www.engineeringtoolbox.com/air-altitude-pressure-d_462.html>
> Another important thing, if your humidity is too high you can keep it below
> 35% safely, but if the region is too dry and you have a drying device
> working and humidity suddenly goes below 30% your possibilities of having
> ESD goes trough the roof, so if you want to be safe, my two cents is that
> you should try to keep humidity in the middle of the range given by the
> factory, far away of the "safety operation limits", because when you are
> walking in the limit, you can easily fall out if it, and the fail when temp
> is higher than asked is similar to the trouble when humidity goes below the
> limit asked, your drying system is not going to hudify back up.
>
>
http://www.esdsystems.com/whitepapers/wp_humidity.html>
> The links have more information on the matters exposed
>
> Gabriel Orozco Hoyuela
>
>
>
> > Date: Thu, 28 Jul 2011 20:39:45 -0600
> > From:
[hidden email]
> > Subject: Re: Incubator box heating mystery
> > To:
[hidden email]
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > *****
> >
> > It's hard to get, but really you want +/-1 degree C and humidity <35%.
> This
> > is particularly important if you have a lot of free-space optics. Most
> > fiber-coupled systems can tolerate some temperature change, but
> free-space
> > beams are guided by steering mirrors. Temperature changes can cause the
> > mirror mounts to flex which misaligns your beam. Additionally, Ti:Saph
> > lasers used in two-photon HATE humidity. We're in Calgary, Canada, which
> is
> > very, very dry in the winter. We can run our lasers without a dry air or
> > nitrogen purge at 925nm. In the summer though, the humidity gets quite
> high
> > and purging becomes necessary. The building contractor just finished
> > installing a humidity control system for our lab last week, so we will
> see
> > how things go. Thus far it seems to be holding the humidity under about
> > 37%. I'll wait a couple weeks and then check my datalogger. @:-)
> >
> > Craig
> >
> >
> > On Thu, Jul 28, 2011 at 6:59 PM, Graham Wright <
> >
[hidden email]> wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > > *****
> > >
> > > I agree with Craig that the temperature and humidity loggers give you
> > > a real insight into what's going on with the aircon in your rooms
> > > (particularly over the weekends in our case). I'd be interested though
> > > in what you think are reasonable limits for the tolerable fluctuations
> > > in both temperature and humidity. We are constantly battling with both
> > > in Singapore!
> > >
> > > Regards,
> > > Graham
> > >
> > > ---
> > > Graham Wright, PhD
> > > Microscopy Unit Manager
> > >
> > > Institute of Medical Biology
> > > 8A Biomedical Grove, #06-06 Immunos, Singapore 138648
> > >
> > > E:
[hidden email]
> > > W:
http://www.imb.a-star.edu.sg/imu/> > >
> > >
> > >
> > > On 29 July 2011 02:51, Craig Brideau <
[hidden email]> wrote:
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > > > *****
> > > >
> > > > We had quite a bit of trouble with our room ventilation until quite
> > > > recently. We purchased an inexpensive USB datalogger to continuously
> > > record
> > > > temperature and humidity in the room. It picked up large swings in
> > > > temperature and humidity that the ventilation system was supposed to
> > > > prevent. The data we collected finally convinced the building
> contractor
> > > to
> > > > install proper humidity controls and hardware for our room. I
> recommend
> > > > that every facility should keep logs of temperature and humidity. The
> > > > loggers are quite cheap now, and can be programmed, detached from the
> > > > computer, and left unattended for months. We would move ours around
> the
> > > > room to get a feel for any gradients, heat loads from equipment, etc.
> > > >
> > > > Craig
> > > >
> > > > On Thu, Jul 28, 2011 at 11:03 AM, Zac Arrac Atelaz <
>
[hidden email]
> > > >wrote:
> > > >
> > > >> *****
> > > >> To join, leave or search the confocal microscopy listserv, go to:
> > > >>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > > >> *****
> > > >>
> > > >>
> > > >> Esteban:
> > > >>
> > > >> Your problem might be the changing position of the termometer of the
> > > >> system, one interesting trial will be putting the overheating system
> in
> > > the
> > > >> non overheating system, to see if is that part fails or remain
> working
> > > as
> > > >> the trial you make the way around.
> > > >>
> > > >> If your system is failing you should consider one incubator as the
> one
> > > we
> > > >> have, this is not the huge box making the microscope unreachable, it
> is
> > > the
> > > >> size of the insert in the stage and it has 4 points heating your
> sample,
> > > the
> > > >> cover, the objective used, and the water heater, we have never had a
> > > >> temperature overshoot as the mentioned by Michael, even if we open
> > > doors, or
> > > >> change room temperature ( recorded from 18 to 27°C) the shift in
> > > temperature
> > > >> observed trough time in the incubator is about 0.5°C over a 25h
> period.
> > > In
> > > >> such long experiments we have people reviewing samples as you dont
> want
> > > to
> > > >> let the confocal working if the sample has suffered unwanted
> changes, by
> > > the
> > > >> way the brand of the one we have is a INU - Tokai hit incubator.
> > > >>
> > > >> I hope this helps a little.
> > > >>
> > > >> Gabriel Orozco Hoyuela
> > > >>
> > > >>
> > > >> > Date: Wed, 27 Jul 2011 12:01:32 -0700
> > > >> > From:
[hidden email]
> > > >> > Subject: Incubator box heating mystery
> > > >> > To:
[hidden email]
> > > >> >
> > > >> > *****
> > > >> > To join, leave or search the confocal microscopy listserv, go to:
> > > >> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > > >> > *****
> > > >> >
> > > >> > Hello everyone,
> > > >> >
> > > >> > I have a very strage problem with an incubator box heating up. I
> have
> > > two
> > > >> > live cell imaging microscopes enclosed in PeCon incubator boxes (I
> > > think
> > > >> > they are Incubator XL). The microscopes are in two separate
> buildings.
> > > >> One
> > > >> > microscope heats up significantly past the set temp. (above 41
> > > degrees,
> > > >> set
> > > >> > to 37 degrees) even though the temp. is being measured and
> reported
> > > >> > correctly to the electronics (confirmed by a glass thermometer).
> The
> > > >> other
> > > >> > microscope works perfectly and holds at 37 degrees for days. The
> > > strange
> > > >> > thing is that when I put the temp. control components that work
> well
> > > >> > (heater, control electronics, temp. sensor, and all cables) onto
> the
> > > >> > microscope that heats up, it still heats up, even though the
> > > components
> > > >> work
> > > >> > perfectly on the other microscope! Any ideas why this might be
> > > happening,
> > > >> > why the temp. control equipment works on one microscope but not on
> the
> > > >> > other?
> > > >> >
> > > >> > The temp. equipment is stand-alone, not connected to a computer or
> to
> > > the
> > > >> > microscope in any way. One microscope is a Zeiss Axiovert 200M
> (the
> > > one
> > > >> > that works) and the other is a Leica DM IRE2 (heats up) in
> separate
> > > >> > buildings; the incubator boxes surrounding them are very similar.
> Any
> > > >> ideas
> > > >> > on this mystery are welcome.
> > > >> >
> > > >> > Thanks,
> > > >> > Esteban
> > > >>
> > > >>
> > > >
> > >
>
>