http://confocal-microscopy-list.275.s1.nabble.com/Settings-adjustment-with-depth-for-a-Zeiss-LSM-510-tp6622398p6645718.html
license number), should be on USB stick on back of computer. If that
doesn't work call your local Zeiss rep. or support at 800-233-2343.
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> We have a Zeiss 510 Meta, and would like to learn more its software too.
> However, the Zeiss FTP site listed below requires an Username and Password.
> How can we access the FTP site?
>
> Shaohui Huang, Ph.D.
> Institute for Environmental Medicine
> UPenn School of Medicine
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
[hidden email]]
> On Behalf Of Jacqui Ross
> Sent: Wednesday, July 27, 2011 8:56 PM
> To:
[hidden email]
> Subject: Re: Settings adjustment with depth for a Zeiss LSM 510
>
> Hi Pedro,
>
> We also have an LSM 510 META. If you have the Guided Tour PDF, page 45
> explains how to use the Auto Z Brightness correction. However, you can only
> set 2 positions (with associated gain/offset) so sometimes this isn't
> enough.
>
> Kind regards,
>
> Jacqui
>
> Jacqueline Ross
>
> Biomedical Imaging Microscopist
> Biomedical Imaging Research Unit
> School of Medical Sciences
> Faculty of Medical & Health Sciences
> The University of Auckland
> Private Bag 92019
> Auckland, NEW ZEALAND
>
> Tel: 64 9 373 7599 Ext 87438
> Fax: 64 9 373 7484
>
>
http://www.fmhs.auckland.ac.nz/sms/biru/>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
[hidden email]]
> On Behalf Of Pedro Almada
> Sent: Wednesday, 27 July 2011 10:25 p.m.
> To:
[hidden email]
> Subject: Re: Settings adjustment with depth for a Zeiss LSM 510
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hi Esteban and Martin,
>
> Thanks alot for the help. I admit I wasn't aware of the option being in the
> software itself. I'll try and have a look at their preparations myself and
> check for oversaturation (if I remember their pictures correctly, that
> sounds like a definite possibility).
>
> Thanks again, this is a very helpful and interesting list.
>
> All the best,
> Pedro
>
> On 26 July 2011 16:37, G. Esteban Fernandez
> <
[hidden email]>wrote:
>
> > The acquisition software (LSM or ZEN) has that function built in, no
> > need for a macro. It is called something like "Z brightness
> > correction" and is in the Z stack panel.
> >
> > Software manuals for LSM and ZEN can be found at the Zeiss FTP site here:
> > ftp://lsm.zeiss.com/LSM/User_Area/Manuals/
> >
> > -Esteban
> >
> > On Tue, Jul 26, 2011 at 7:26 AM, Pedro Almada <
[hidden email]> wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> >> *****
> >>
> >> Dear listers,
> >>
> >> Does anyone know of a VBA macro for the Zeiss Meta which allows users
> >> to adjust settings such as gain and laser power according to current
> >> focus for the Zeiss LSM 510? I am interested in developing this
> >> myself but if there is something already made, that would be great...
> >>
> >> If you are curious what it's for, it's for some of our users who
> >> image whole drosophila brains for structural information. Their
> >> common complaint is that half-way through their z-stack they loose
> >> image brightness until the last slices are barely visible. Clearing
> >> methods like Methyl salicilate aren't really adequate for neuronal
> >> structure I'm told, which leads me to consider this method.
> >>
> >> Either way, any information is welcome.
> >>
> >> Thanks for reading,
> >> Cheers,
> >> Pedro Almada
> >> *
> >> *
> >> Research and Microscopy Technician
> >> Unidade de Imagiologia Celular,
> >> *Instituto Gulbenkian de Ciência*
> >> Rua da Quinta Grande, 6
> >> 2780-156 Oeiras
> >>
> >> Phone: + 351 214 464 607
> >> Ext: 607
> >>
> >
>