Uniformity of 2-photon illumination?

Posted by stu_the_flat on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Uniformity-of-2-photon-illumination-tp6719119.html

Dear list users,

We have a Zeiss 510 two-photon system, it seems to have a an non-uniform field of illumination,

I have tried to test this by imaging a chroma fluorescent test slide there is a two fold drop in fluorescent signal across the X axis, the there appears to be a slight variation on the Y axis as well,

What is most likely to be causing this? Is imaging a test slide a fair test?

Any advice would be most welcome.

Thank you

Stuart McIntyre