Re: Uniformity of 2-photon illumination?

Posted by Vladimir Ghukasyan-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Uniformity-of-2-photon-illumination-tp6719119p6719142.html

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Stuart,

Most probably you see a result of a bad aligned laser beam in the scanning unit.

Best wishes,
Vladimir


On Wed, Aug 24, 2011 at 3:14 AM, stu_the_flat <[hidden email]> wrote:

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> Dear list users,
>
> We have a Zeiss 510 two-photon system, it seems to have a an non-uniform
> field of illumination,
>
> I have tried to test this by imaging a chroma fluorescent test slide there
> is a two fold drop in fluorescent signal across the X axis, the there
> appears to be a slight variation on the Y axis as well,
>
> What is most likely to be causing this? Is imaging a test slide a fair test?
>
> Any advice would be most welcome.
>
> Thank you
>
> Stuart McIntyre
>
>
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