Posted by
Guy Cox-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Uniformity-of-2-photon-illumination-tp6719119p6719167.html
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The Chroma slide should be a pretty good test. You should do 2 or 3
focal positions in case the effect is actually a tilt in the stage or
the optics. Do you see the same effect in the confocal and
non-descanned detectors? If so it's clearly not something interfering
with the detection optics, and you'd have to blame the illumination.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
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On Behalf Of stu_the_flat
Sent: Wednesday, 24 August 2011 5:14 PM
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Subject: Uniformity of 2-photon illumination?
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Dear list users,
We have a Zeiss 510 two-photon system, it seems to have a an non-uniform
field of illumination,
I have tried to test this by imaging a chroma fluorescent test slide
there
is a two fold drop in fluorescent signal across the X axis, the there
appears to be a slight variation on the Y axis as well,
What is most likely to be causing this? Is imaging a test slide a fair
test?
Any advice would be most welcome.
Thank you
Stuart McIntyre
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