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Thank you very much for your replies.
Just to clear up a few points, I always take Z sections of the slide just to make sure it wasn’t a case that the slide was at an angle, (The system is not designed to hold slides so its always a bit “Heath Robison” trying to get a slide underneath it!)
I had checked various sites around the slide and I was getting consistent results, yesterday I scanned the slide with the descanned detectors and got a similar image.
We want all the power we can get as would love to be able to image more deeply into cardiac muscle, (is it a fair assumption that more power will allow us to image deeper?) for that reason we have a Coherent Chameleon Ultra II, and I believe its peak power is around 5.5W. However would the alignment not take place “down stream” of the OPO? So I could just set that to its minimum intensity setting.
Anyway it is kind of irrelevant because the system is under service contract with Zeiss, So all I need to do it get on the phone and start whining at them, Although I would love to give laser alignment a go myself!
Once again thank you for all your replies
Yours Stuart McIntyre |
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