Posted by
Kilgore, Jason-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Asante-Red-Calcium-tp6746843p6750961.html
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Thomas,
I don't know this dye, so I can't be sure, but are you certain you don't need to cleave the AM group for it to become active?
Jason
** no corporate affiliation with TefLabs **
Jason A. Kilgore
Technical Application Scientist
Molecular Probes Labeling and Detection Technologies
Cells Systems Division
T 1 800 955 6288 then option 5 or 541 335 0353 . F 541 335 0238
29851 Willow Creek Rd . Eugene . OR . 97402-9132 . United States
www.invitrogen.com/technicalsupport
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Thomas Volksdorf
Sent: Thursday, September 01, 2011 5:18 AM
To:
[hidden email]
Subject: Re: Asante Red Calcium
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Hello Tom and everyone else who might be interested in the ACaR-dye,
I am currently trying to establish this dye in our laboratory. I've ordered
the 10 x 50 µg kit of the AM-dye and try to load keratinocytes with it. I
already managed to get some pictures using our 2-photon-microscope, but I'm
not really satisfied yet because the signal for the unloaded dye (that you
need to calculate the ratiometrics) is very very week, but on the other hand
seems to have a high background noise. Using enough laserpower to actually
get a signal leads to significant bleaching and cell destruction...
I have to try a different PMT-setup yet, and a colleague of mine told me to
try imaging the cells with a CCD-Camera , so that I don't have to worry
whether one PMT might be more responsive or something like this. I'll
hopefully have the time do these tests this month, and I can provide status
updates if you like.
Kind regards,
Thomas