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Mathew,
The book Fundamentals of Light Microscopy and electronic imaging by Douglas B. Murphy
has a nice experimental bench-top confocal on page 212
But it doesn't involve either scan mirrors or a spinning disk.
--
Paul Herzmark
Specialist
[hidden email]Department of Molecular and Cell Biology
479 Life Science Addition
University of California, Berkeley
Berkeley, CA 94720-3200
(510) 643-9603
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---------- Forwarded message ----------
From:
Matthew Nicholas <[hidden email]>
Date: Fri, Aug 8, 2008 at 9:40 AM
Subject: confocal for a teaching lab
To:
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Hello,
I am helping to prepare a set of labs for a graduate course on biological light microscopy. For a few of the labs, we are interested in constructing a confocal microscope, to show students how it works, and to use in imaging cells. Specifically, we're interested in the nipkow disk-scanning type (though we are still considering some designs using conventional scanning with galvos).
I am wondering if anyone with experience doing this has any words of advice? Also, I would be interested to know if anyone has or knows of an old or broken confocal that we might be able to purchase at a bargain either use for parts or repair.
Thanks in advance,
Matt Nicholas
MSTP III
Albert Einstein College of Medicine