Posted by
Daniel James White on
URL: http://confocal-microscopy-list.275.s1.nabble.com/10x-objectives-for-multiphoton-work-tp6823281.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
Hi Adrian,
Zeiss have a water immersion 10x / 0.45
ziess objective database says its 65% at 1000 nm.
C-Apochromat 10x/0.45 W M27
it expects a 0.17 coverglass....so you must look through a window....but
1.8 mm Working distance...
didnt try it for 2 photon yet.... we have it on a widefield system.
Dan
On Sep 23, 2011, at 7:02 AM, CONFOCALMICROSCOPY automatic digest system wrote:
>
> Date: Thu, 22 Sep 2011 17:13:54 +1000
> From: Adrian Smith <
[hidden email]>
> Subject: 10x objectives for multiphoton work?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=3Dconfocalmicroscopy> *****
>
> Hi all,
>
> Just wondering if anyone has experience with any 10x objectives for =
> intravital MP work?
>
> Not fussy about manufacturer at this point - just looking for =
> recommendations.=20
>
> (e.g. I can see 10x NA0.45 Plan Apo objectives in the Nikon and (maybe) =
> Zeiss catalogs=85 and there is even a NA0.5 Fluor in those as well).
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
Dr. Daniel James White BSc. (Hons.) PhD
Leader - Image Processing Facility,
Senior Microscopist,
Light Microscopy Facility.
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany
+49 (0)15114966933 (German Mobile)
+49 (0)351 210 2627 (Work phone at MPI-CBG)
+49 (0)351 210 1078 (Fax MPI-CBG LMF)
chalkie666 Skype
http://www.bioimagexd.net BioImageXD
http://fiji.sc Fiji - is just ImageJ (Batteries Included)
http://www.chalkie.org.uk Dan's Homepages
https://ifn.mpi-cbg.de Biopolis Dresden Imaging Platform (BioDIP)
dan (at) chalkie.org.uk
( white (at) mpi-cbg.de )