Re: Image Processing Software

Posted by Daniel James White on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Image-Processing-Software-tp6821785p6823375.html

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Hi Venkat,

On Sep 23, 2011, at 7:02 AM, CONFOCALMICROSCOPY automatic digest system wrote:

> Date:    Thu, 22 Sep 2011 16:23:42 -0400
> From:    John Oreopoulos <[hidden email]>
>
> Try ImageJ. It's free:
>
> http://rsbweb.nih.gov/ij/

And if you like ImageJ, you should really really like Fiji,
since its just imageJ, but with a bunch of nice stuff added to make it easier to use, update and script etc.

http://fiji.sc        Fiji -  is just ImageJ (Batteries Included)

Its made by the same folks who are making ImageJ2, LOCI's Bio-formats and lots of cool plugind bundled with Fiji.
Many Fiji features already got absorbed into ImageJ, and even more will be in imagej2.

For a facility, open platforms make a lot of sense, from efficient and practical standpoints.
Also the users are given more transferable skills which they can take anywhere.
Conversely, if users are trained in a closed platform, then they are married to that,
and are in trouble when they move on and don't have a license for that software, and worse cant afford one.

That said, for Deconvolution, Huygens is very very good,
Imaris has some nice features, as do Volocity, and other expensive softwares...
but if I can train a user to do what they want
in Fiji, or cell profiler, etc.,  and later R for analysis instead of Matlab or SPSS, then thats best all round.  

I think its good to use the resources that you would otherwise spend on expensive license to hire a programmer to contribute
useful new tools to the community, as an Im,ageJ/Fiji plugin or similar.
Our facility users always want to do something special that no software can currently do easily,
and it takes years for commercial vendors to come up with the little feature you desperately need,
so its more efficient to do it yourself - and the whole community also gets the fruit of your labour
and its publishable and citeable!


cheers

Dan



>
>
> On 2011-09-22, at 11:39 AM, Ramshesh, Venkat K wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>> =20
>> Dear all,
>> =20
>> Our imaging core is looking to buy imaging software for offline image =
> processing, quantification etc. for mostly confocal and multiphoton =
> fluorescence microscopy. I was hoping if folks here had any suggestions =
> or recommendations as to which software to purchase in this regard. =
> Thanks for all your help in advance.
>> =20
>> Best,
>> Venkat
>> =20
>> Venkat Ramshesh, PhD
>> Research Instructor/Facility Manager
>> Department of Pharmaceutical and Biomedical Scinces and Hollings =
> Cancer Center
>> Drug Discovery Bldg. DD 507
>> 70 President Street, MSC 140
>> MUSC, Charleston, SC 29425
>> Ph: 8438762363
>> Fax:8438762353
>> E-mail: [hidden email]

Dr. Daniel James White BSc. (Hons.) PhD

Leader - Image Processing Facility,
Senior Microscopist,
Light Microscopy Facility.

Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany

+49 (0)15114966933 (German Mobile)
+49 (0)351 210 2627 (Work phone at MPI-CBG)
+49 (0)351 210 1078 (Fax MPI-CBG LMF)
chalkie666 Skype
http://www.bioimagexd.net  BioImageXD
http://fiji.sc                                        Fiji -  is just ImageJ (Batteries Included)
http://www.chalkie.org.uk                Dan's Homepages
https://ifn.mpi-cbg.de  Biopolis Dresden Imaging Platform (BioDIP)
dan (at) chalkie.org.uk
( white (at) mpi-cbg.de )