C. elegans imaging

Posted by Claire Brown on
URL: http://confocal-microscopy-list.275.s1.nabble.com/C-elegans-imaging-tp6826291.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****


We have a lab that wants to screen a C. Elegans genetic library with about 18,000 genes. They want to take images of the worms on 24 well plates and measure the distance between 2 of 3 EGFP spots on the worms.

We would like to do the experiments on an inverted HCS automated microscope. The problem is they have the worms growing on a ~1 mm thick nutrient-rich agar layer.

We need to image the worms through the agar so we need to find a different way to prepare the samples. Has anyone ever tried something like this? If so do you have a solution for this? The agar is poured by hand so getting it much thinner than 1 mm would be difficult.


Sincerely,

Claire

--------------------------------------------------------------------------------
Claire M. Brown, PhD
McGill University
Life Sciences Complex Imaging Facility Director