Posted by
Jeremy Adler-4 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/C-elegans-imaging-tp6826291p6826500.html
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1 mm agar plus a 1mm+ plastic base are not ideal imaging conditions. A
long working distance objective will focus but the thick plastic base
will degrade the image - most objectives are designed for 0.17mm
glass. But how good do the images need to be for your purposes ?
A solution is glass bottomed multiwell plates, but they are relatively
expensive. Given the popularity of multiwell plates for imaging there
is probably a decent market for a plate with a suitably thin plastic
bottom.
Quoting "Claire Brown, Dr." <
[hidden email]>:
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>
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>
> We have a lab that wants to screen a C. Elegans genetic library with
> about 18,000 genes. They want to take images of the worms on 24 well
> plates and measure the distance between 2 of 3 EGFP spots on the
> worms.
>
> We would like to do the experiments on an inverted HCS automated
> microscope. The problem is they have the worms growing on a ~1 mm
> thick nutrient-rich agar layer.
>
> We need to image the worms through the agar so we need to find a
> different way to prepare the samples. Has anyone ever tried
> something like this? If so do you have a solution for this? The agar
> is poured by hand so getting it much thinner than 1 mm would be
> difficult.
>
>
> Sincerely,
>
> Claire
>
> --------------------------------------------------------------------------------
> Claire M. Brown, PhD
> McGill University
> Life Sciences Complex Imaging Facility Director
>
Jeremy Adler
IGP
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