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> We have a lab that wants to screen a C. Elegans genetic library with about
> 18,000 genes. They want to take images of the worms on 24 well plates and
> measure the distance between 2 of 3 EGFP spots on the worms.
>
> We would like to do the experiments on an inverted HCS automated
> microscope. The problem is they have the worms growing on a ~1 mm thick
> nutrient-rich agar layer.
>
> We need to image the worms through the agar so we need to find a different
> way to prepare the samples. Has anyone ever tried something like this? If so
> do you have a solution for this? The agar is poured by hand so getting it
> much thinner than 1 mm would be difficult.
>
>
> Sincerely,
>
> Claire
>
>
> --------------------------------------------------------------------------------
> Claire M. Brown, PhD
> McGill University
> Life Sciences Complex Imaging Facility Director
>