Re: Image Processing Software

Posted by Scott, Mark on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Image-Processing-Software-tp6821785p6831668.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi Venkat,

As many have already suggested ImageJ (or FIJI) is a very nice option since it will do almost anything you could require of it and it is free.   However, it is a rather cut down UI typical of open source software and as such isn't the most user friendly of available software - and as it is for a facility it is important that your users feel comfortable with using the software (yes it might be feasible for you and I to learn how to do something but not all microscope users are as comfortable with this thought).

Other generalised software would include Volocity and Imaris, both very nicely packaged.   There are also of course a lot of other software solutions out there designed around specific analysis problems such as Autoquant, Definiens (which is working with OMERO importation now afaik) and many others.

I guess one thing to do is assess what the users of your facility are likely to need (or currently need) and go from there.  There is little sense in buying an expensive package in for a specific purpose for only a single user when ImageJ may do it and it isn't too bad to ask one user to learn it, but likewise if a lot of users want a similar feature then it becomes more important to assess ease of use as well as cost.  No sense havign free software that is never used due to uses being scared of it.

Cheers


Mark Scott

FILM - Facility for Imaging by Light Microscopy
Senior Research Technician
Sir Alexander Fleming Building, desk 408
Imperial College London / South Kensington
Exhibition Road
London SW7 2AZ
UK
Tel: ++44(0)20-759-49793
E-mail: [hidden email]
Website: http://imperial.ac.uk/imagingfacility 



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ramshesh, Venkat K
Sent: 22 September 2011 16:39
To: [hidden email]
Subject: Image Processing Software

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear all,

Our imaging core is looking to buy imaging software for offline image processing, quantification etc. for mostly confocal and multiphoton fluorescence microscopy. I was hoping if folks here had any suggestions or recommendations as to which software to purchase in this regard. Thanks for all your help in advance.

Best,
Venkat

Venkat Ramshesh, PhD
Research Instructor/Facility Manager
Department of Pharmaceutical and Biomedical Scinces and Hollings Cancer Center
Drug Discovery Bldg. DD 507
70 President Street, MSC 140
MUSC, Charleston, SC 29425
Ph: 8438762363
Fax:8438762353
E-mail: [hidden email]