Re: confocal for a teaching lab

Posted by Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/confocal-for-a-teaching-lab-tp681103p683755.html

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You'd really have to buy the Nipkow disk - pretty hard to make.

The 'easy' way to set up a confocal microscope on an optical table

would be to use stage scanning - either with stepper motors or

by using the Colin Sheppard technique of using the voice

coils from small speakers.

 

                                                                       Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
    http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone [phone]     Fax [phone]
Mobile [phone]
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     http://www.guycox.net

 

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Paul Herzmark
Sent: Saturday, 9 August 2008 3:22 AM
To: [hidden email]
Subject: Fwd: confocal for a teaching lab

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Mathew,
The book Fundamentals of Light Microscopy and electronic imaging by Douglas B. Murphy
has a nice experimental  bench-top confocal on page 212

But it doesn't involve either scan mirrors or a spinning disk.


--
Paul Herzmark
Specialist
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Department of Molecular and Cell Biology
479 Life Science Addition
University of California, Berkeley
Berkeley, CA 94720-3200
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---------- Forwarded message ----------
From: Matthew Nicholas <[hidden email]>
Date: Fri, Aug 8, 2008 at 9:40 AM
Subject: confocal for a teaching lab
To: [hidden email]


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Hello,
I am helping to prepare a set of labs for a graduate course on biological light microscopy. For a few of the labs, we are interested in constructing a confocal microscope, to show students how it works, and to use in imaging cells. Specifically, we're interested in the nipkow disk-scanning type (though we are still considering some designs using conventional scanning with galvos).

I am wondering if anyone with experience doing this has any words of advice? Also, I would be interested to know if anyone has or knows of an old or broken confocal that we might be able to purchase at a bargain either use for parts or repair.

Thanks in advance,
Matt Nicholas

MSTP III
Albert Einstein College of Medicine

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