Re: 10x objectives for multiphoton work?

Posted by Zac Arrac Atelaz on
URL: http://confocal-microscopy-list.275.s1.nabble.com/10x-objectives-for-multiphoton-work-tp6823281p6840634.html

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There is a 25x water inmersion in Olympus, with 82% transmittance from 400nm to 1000nm, so you can imagine that this can easily can go down about 1mm or so, on a regular basis, this one is designed specially for deep imaging and 2P, there is some transparent embryo technique that can achieve even more thant that
 
Regards
 
Gabriel OH
 

> Date: Tue, 27 Sep 2011 05:24:23 -0500
> From: [hidden email]
> Subject: Re: 10x objectives for multiphoton work?
> To: [hidden email]
>
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> There is no 10X N: 0.45 water immersion Zeiss. I used it on a 2P. It is really
> good but less than the 20X water immersion. With which we have obtained
> more than 980micron deep in adult mouse brain (Dextran-marking rhoamine).
> Be careful not to put water on the front otherwise very poor results. There is
> a 10X water immersion but really not very good.