Re: looking for "beautiful" sample

Posted by Zac Arrac Atelaz on
URL: http://confocal-microscopy-list.275.s1.nabble.com/looking-for-beautiful-sample-tp6850345p6855575.html

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I suggest also plants, transversal cut of the stem will be amazing, and there are old school dyes that have differential fluorescence also.
 
Gabriel OH
 

> Date: Mon, 3 Oct 2011 16:35:22 +0200
> From: [hidden email]
> Subject: Re: looking for "beautiful" sample
> To: [hidden email]
>
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> >
> >
> > Dear all,
> > here it is, a silly question. I wish to demonstrate the
> > capabilities of
> > our new system that can read-out fluorescence lifetime, anisotropy and
> > spectra wih TPE. I have plenty of information-rich images, either
> > images
> > from tissue or fluorescent proteins, but I would like to prepare an
> > eye-catching set of images.
> >
> > I am a bit bored to use Convallaria... any suggestion for a
> > fluorescent or
> > autofluorescent sample tha could provide a relative complex emission
> > that
> > could look good?
>
> Hi Alessandro,
>
> Bouncing on Cathy's advice, I strongly encourages you to try the
> pollen, though in my hemisphere, it is no longer the adequate season.
> They are autofluorescent in a very wide range of wavelengths, have
> awesome 3D structure. On top of that, meta-scientifically:
> - It is a fantastic hobby to do with your kids: go out with them with
> slides, coverslips, oil and nail-polish and go collect pollen from the
> flowers of your garden
> - Since it is not the season, it is a good opportunity to offer
> flowers to your wife. In my case however, I did not date bringing the
> bill to the lab.
>
> Best regards
> jy
>
>
>
> --
> Jean-Yves Tinevez
> PFID - Imagopole
> Institut Pasteur
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> 75724 Paris cedex 15
> France
> tel: +33 1 40 61 35 40