Posted by
John Oreopoulos on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Advice-on-citations-for-homogenized-fluorescence-illumination-tp6854775p6855683.html
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There's one other reference I remembered written back in 2003 that summarizes quite nicely the state of the art at that time for regular, wide-field epifluorescence microscopy:
1. Dimas, C.F., J.J. Kuta, and M. Hubert, An enhanced method of obtaining uniform excitation radiation for fluorescence microscopy, in Applications of Photonic Technology 6 - Closing the Gap between Theory, Development, and Application, R.A. Lessard and G.A. Lampropoulos, Editors. 2003, Spie-Int Soc Optical Engineering: Bellingham. p. 558-567.
And in the context of TIRF microscopy, we've discussed this topic before on the confocal list server. See here:
http://lists.umn.edu/cgi-bin/wa?A2=ind1011&L=CONFOCALMICROSCOPY&D=0&P=18312The relevant articles are:
2. Fiolka, R., et al., Even illumination in total internal reflection fluorescence microscopy using laser light. Microscopy Research and Technique, 2008. 71(1): p. 45-50.
3. Mattheyses, A.L., K. Shaw, and D. Axelrod, Effective elimination of laser interference fringing in fluorescence microscopy by spinning azimuthal incidence angle. Microscopy Research and Technique, 2006. 69(8): p. 642-647.
4. van't Hoff, M., V. de Sars, and M. Oheim, A programmable light engine for quantitative single molecule TIRF and HILO imaging. Optics Express, 2008. 16(22): p. 18495-18504.
John Oreopoulos
Research Assistant
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca
On 2011-10-03, at 10:52 AM, Chen, De (NIH/NCI) [C] wrote:
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> Hi Ricardo:
>
> The non-uniform illuminaiton can be corrected post-acquisition which can be done by the polynomial fitting to each row of the image intensity profile and followed by a subtraction.
>
>
> ________________________________________
> From: Ricardo Henriques [
[hidden email]]
> Sent: Monday, October 03, 2011 8:12 AM
> To:
[hidden email]
> Subject: Advice on citations for homogenized fluorescence illumination
>
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> Hello everyone,
>
> I'm preparing a small manuscript that in part covers the concept that most fluorescence microscopes (SD, WF, TIRF, ...) have a non-homogenous illumination of the field of view - typically gaussian-shaped, less intensity on the image extremities. I was wondering if you could advise me on what you consider the most interesting publications tackling or approaching this subject.
>
> Thank you in advanced.
> Best regards,
> Ricardo Henriques
>
> Ricardo Henriques
> Instituto de Medicina Molecular (Lisbon, Portugal).
> For contact information see:
https://sites.google.com/site/paxcalpt/