http://confocal-microscopy-list.275.s1.nabble.com/Advice-on-citations-for-homogenized-fluorescence-illumination-tp6854775p6855763.html
This is extremely useful. I'm grabbing now the literature and will check it out.
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> There's one other reference I remembered written back in 2003 that summarizes quite nicely the state of the art at that time for regular, wide-field epifluorescence microscopy:
>
> 1. Dimas, C.F., J.J. Kuta, and M. Hubert, An enhanced method of obtaining uniform excitation radiation for fluorescence microscopy, in Applications of Photonic Technology 6 - Closing the Gap between Theory, Development, and Application, R.A. Lessard and G.A. Lampropoulos, Editors. 2003, Spie-Int Soc Optical Engineering: Bellingham. p. 558-567.
>
> And in the context of TIRF microscopy, we've discussed this topic before on the confocal list server. See here:
>
>
http://lists.umn.edu/cgi-bin/wa?A2=ind1011&L=CONFOCALMICROSCOPY&D=0&P=18312>
> The relevant articles are:
>
> 2. Fiolka, R., et al., Even illumination in total internal reflection fluorescence microscopy using laser light. Microscopy Research and Technique, 2008. 71(1): p. 45-50.
> 3. Mattheyses, A.L., K. Shaw, and D. Axelrod, Effective elimination of laser interference fringing in fluorescence microscopy by spinning azimuthal incidence angle. Microscopy Research and Technique, 2006. 69(8): p. 642-647.
> 4. van't Hoff, M., V. de Sars, and M. Oheim, A programmable light engine for quantitative single molecule TIRF and HILO imaging. Optics Express, 2008. 16(22): p. 18495-18504.
>
> John Oreopoulos
> Research Assistant
> Spectral Applied Research
> Richmond Hill, Ontario
> Canada
> www.spectral.ca
>
> On 2011-10-03, at 10:52 AM, Chen, De (NIH/NCI) [C] wrote:
>
>> *****
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>>
>> Hi Ricardo:
>>
>> The non-uniform illuminaiton can be corrected post-acquisition which can be done by the polynomial fitting to each row of the image intensity profile and followed by a subtraction.
>>
>>
>> ________________________________________
>> From: Ricardo Henriques [
[hidden email]]
>> Sent: Monday, October 03, 2011 8:12 AM
>> To:
[hidden email]
>> Subject: Advice on citations for homogenized fluorescence illumination
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> *****
>>
>> Hello everyone,
>>
>> I'm preparing a small manuscript that in part covers the concept that most fluorescence microscopes (SD, WF, TIRF, ...) have a non-homogenous illumination of the field of view - typically gaussian-shaped, less intensity on the image extremities. I was wondering if you could advise me on what you consider the most interesting publications tackling or approaching this subject.
>>
>> Thank you in advanced.
>> Best regards,
>> Ricardo Henriques
>>
>> Ricardo Henriques
>> Instituto de Medicina Molecular (Lisbon, Portugal).
>> For contact information see:
https://sites.google.com/site/paxcalpt/