Re: co-planar localization

Posted by Beat Ludin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/co-planar-localization-tp677332p685581.html

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At 08:39 07-08-2008, <[hidden email]> wrote:
>Colocalisation based on the coincidence of colors in
>still images is always limited by the spatial resolution.

Keep in mind though that resolution is commonly defined as the
minimum distance at which to objects of the same color in an image
can be discerned.
However, the relative position of objects of different colors can be
determined with much higher precision because their intensity
profiles can be analyzed individually (provided that the S/N is
sufficient and that chromatic abberrations of the system are accounted for).

Beat