Re: looking for "beautiful" sample

Posted by Gregg Sobocinski on
URL: http://confocal-microscopy-list.275.s1.nabble.com/looking-for-beautiful-sample-tp6850345p6856576.html

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Alessandro,
I've been meaning to purchase something more interesting, myself. From a cost-value point of view, Carolina Biological has an excellent selection. I've been wanting to purchase a mixed pollen slide from them, but I didn't see it with my initial, quick look today.

http://www.carolina.com/category/30000/30800.do

Plants autofluoresce wonderfully. If you want to separate the channels, you'll want to be more specific, though. I've seen Lily anther's used to great effect.

When I train users on the confocal microscope, I use a Molecular Probes cell slide#2. At $130 it's pricey, but I get a DAPI, Bodipy, and Texas Red slide, which offers the challenges of triple-staining, making training that much more effective.

Good luck,
~Gregg

Gregg Sobocinski
Microscope Imaging Specialist
University of Michigan
Molecular, Cellular, and Developmental Biology
Ann Arbor, Michigan
USA


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alessandro Esposito
Sent: Saturday, October 01, 2011 6:10 AM
To: [hidden email]
Subject: looking for "beautiful" sample

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Dear all,
    here it is, a silly question. I wish to demonstrate the capabilities of
our new system that can read-out fluorescence lifetime, anisotropy and
spectra wih TPE. I have plenty of information-rich images, either images
from tissue or fluorescent proteins, but I would like to prepare an
eye-catching set of images.

I am a bit bored to use Convallaria... any suggestion for a fluorescent or
autofluorescent sample tha could provide a relative complex emission that
could look good?

Cheers,

Alessandro