Posted by
Monique Vasseur on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Advice-on-citations-for-homogenized-fluorescence-illumination-tp6854775p6862044.html
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Hi Chen
What do you mean by polynomial fitting? What is your formula? Thanks in advance.
Monique Vasseur
Microscopie et imagerie
Département de biochimie
Université de Montréal
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De : Confocal Microscopy List [mailto:
[hidden email]] De la part de Chen, De (NIH/NCI) [C]
Envoyé : 3 octobre 2011 10:53
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Objet : Re: Advice on citations for homogenized fluorescence illumination
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Hi Ricardo:
The non-uniform illuminaiton can be corrected post-acquisition which can be done by the polynomial fitting to each row of the image intensity profile and followed by a subtraction.
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From: Ricardo Henriques [
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Sent: Monday, October 03, 2011 8:12 AM
To:
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Subject: Advice on citations for homogenized fluorescence illumination
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Hello everyone,
I'm preparing a small manuscript that in part covers the concept that most fluorescence microscopes (SD, WF, TIRF, ...) have a non-homogenous illumination of the field of view - typically gaussian-shaped, less intensity on the image extremities. I was wondering if you could advise me on what you consider the most interesting publications tackling or approaching this subject.
Thank you in advanced.
Best regards,
Ricardo Henriques
Ricardo Henriques
Instituto de Medicina Molecular (Lisbon, Portugal).
For contact information see:
https://sites.google.com/site/paxcalpt/