Posted by
tineke vendrig on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Advice-on-citations-for-homogenized-fluorescence-illumination-tp6854775p6862104.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
For a homogenous illumination you can re-install the lasers and perform
a Köhler illumination again for the best illumination. Köhler illumination
acts to generate an extremely even illumination of the sample and ensures
that an image of the illumination source is not visible in the
resulting image. Köhler illumination is the predominant technique for sample
illumination in modern scientific light microscopy although it requires
additional optics which less expensive and simpler light microscopes.
Good luck!
Tineke Vendrig, ing
technical engeneer optical microscopy
Delft University of Technology
Bionano Science
Kavli Institute of Nanoscience
Lorentzweg 1
2628LJ Delft
room F185
Tel: +31 27 89299
Fax:+31 15 2781202
2011/10/3 Ricardo Henriques <
[hidden email]>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hello everyone,
>
> I'm preparing a small manuscript that in part covers the concept that most
> fluorescence microscopes (SD, WF, TIRF, ...) have a non-homogenous
> illumination of the field of view - typically gaussian-shaped, less
> intensity on the image extremities. I was wondering if you could advise me
> on what you consider the most interesting publications tackling or
> approaching this subject.
>
> Thank you in advanced.
> Best regards,
> Ricardo Henriques
>
> Ricardo Henriques
> Instituto de Medicina Molecular (Lisbon, Portugal).
> For contact information see:
https://sites.google.com/site/paxcalpt/>