Posted by
Bertrand Vernay-3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslips-tp6865241p6865281.html
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Hi Simon
I used coverslip from Zeiss 170 +/- 5 um (not for super resolution though)
http://www.meditec.zeiss.com/4125681F004CA025/Contents-Frame/C66A2521E524C891852575A200721A7C
Bertrand
--
Bertrand Vernay, PhD
Microscopy Senior Research Associate
Developmental Biology Unit
UCL Institute of Child Health
30 Guilford Street
London WC1N 1EH, UK
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https://www.ucl.ac.uk/ich/services/lab-services/cmcf-----Original Message-----
From: Confocal Microscopy List [mailto:
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Behalf Of Simon Walker
Sent: 06 October 2011 09:43
To:
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Subject: Coverslips
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Hello,
Not strictly a confocal question, but I'm sure someone out there will be
able to help. We are about to start using a SIM super res system and have
been advised that one of the critical factors in acquiring optimal images is
the cover glass. Specifcally, the thickness of the glass needs to be
consistant across the whole coverslip (e.g. 170 um +/- 2 um), and
reproducible between coverslips. One option is to measure each individual
coverslip before use, but this seems rather impractical to me. Has anyone
looked into this, and if so, are there any manufacturers out there who can
provide cover glass with this high specification?
Thanks,
Simon