Posted by
Axel Kurt Preuss on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslips-tp6865241p6865291.html
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I think Marienfeld does. I have ordered them, so not tried yet. But they seem to guarantee tight specs
Axel CIF, IMCB
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From: Confocal Microscopy List [mailto:
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Sent: Thursday, 6 October, 2011 4:48 PM
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Subject: Re: Coverslips
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Hi Simon
I used coverslip from Zeiss 170 +/- 5 um (not for super resolution though)
http://www.meditec.zeiss.com/4125681F004CA025/Contents-Frame/C66A2521E524C891852575A200721A7C
Bertrand
--
Bertrand Vernay, PhD
Microscopy Senior Research Associate
Developmental Biology Unit
UCL Institute of Child Health
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https://www.ucl.ac.uk/ich/services/lab-services/cmcf-----Original Message-----
From: Confocal Microscopy List [mailto:
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Sent: 06 October 2011 09:43
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Subject: Coverslips
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Hello,
Not strictly a confocal question, but I'm sure someone out there will be able to help. We are about to start using a SIM super res system and have been advised that one of the critical factors in acquiring optimal images is the cover glass. Specifcally, the thickness of the glass needs to be consistant across the whole coverslip (e.g. 170 um +/- 2 um), and reproducible between coverslips. One option is to measure each individual coverslip before use, but this seems rather impractical to me. Has anyone looked into this, and if so, are there any manufacturers out there who can provide cover glass with this high specification?
Thanks,
Simon
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