Re: Coverslips

Posted by Julien Cau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslips-tp6865241p6865294.html

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Hi Simon,

We are using Marienfeld glass coverslip #1.5 H.
The tolerance is not 20um as the normal #1.5 category is, but 5um.
To my knowledge, this is the best tolerance.

Reference are :
#0117500 : 12mm round coverslips
#0117580 : 18mm round
# 0107052 : 22x22mm square
#0107222 : 24x50mm

You can get some square 18x18mm from Zeiss as well, but these coverslips
are from Marienfeld anyway.
The french coverslip market is a bit weird as among 40 reference in any
provider's catalog, only 2 are #1.5
I have to request these Marienfeld reference to my coverslip provider as
they are not available in their catalog.

The difference compared to the wrong #1 coverslip is amazing, and
compared to regular #1.5 is quite noticeable.
The price is roughly twice as expensive compared to regular #1.5, but we
are talking about a few euro cents per coverslip, so its better not
wasting a hundreds of euros experiment with crap coverslips.

We have a precision ruler for measuring the thickness of the coverslips,
but you don't want to waste your time doing this do you?

These coverslips are the one we used for our OMX SIM microscope and they
are of course fine. Another crucial parameter is the refraction index
potential mismatch between immersion medium and mounting medium, but I
am sure you are aware of this.
Hope it helped.
Best regards
Julien


Le 06/10/2011 10:43, Simon Walker a écrit :

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>
> Hello,
> Not strictly a confocal question, but I'm sure someone out there will be able to
> help.  We are about to start using a SIM super res system and have been
> advised that one of the critical factors in acquiring optimal images is the cover
> glass.  Specifcally, the thickness of the glass needs to be consistant across
> the whole coverslip (e.g. 170 um +/- 2 um), and reproducible between
> coverslips.  One option is to measure each individual coverslip before use, but
> this seems rather impractical to me.  Has anyone looked into this, and if so,
> are there any manufacturers out there who can provide cover glass with this
> high specification?
> Thanks,
> Simon
>

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*/Julien Cau, PhD./*

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