Rejected posting to CONFOCALMICROSCOPY@LISTS.UMN.EDU

Posted by Hanna_SN on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Rejected-posting-to-CONFOCALMICROSCOPY-LISTS-UMN-EDU-tp6866064.html

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Dear all,

For a couple of months we are struggling with a serious problem with
the field illumination in our Olympus Scan^R Scanning station. The
image of the specimen, when observed on the computer screen is much
darker on one side than on the other. We thought it is the lamp but
the lamp is new at properly inserted. Then we tried calibrating the
optic fiber, but it didn't help at all. Our third guess was the
filters.We checked all of the filters  -  the coating has lots of
small dark spots with biggest concentration of the spots in the middle
of the filters, but I am concerned if that can  produce uneven
illumination of the field so that only one part (let say left corner)
is underilluminated? So, I turn to you and your experience: did may
anyone have the similar problem? Do you have any suggestion what can
be the cause of the problem?

Best regards,
Hanna

--
Department of Plant Anatomy and Cytology
Faculty of Biology and Environmental Protection
University of Silesia
Jagiellonska Str. 28
40-032 Katowice
Poland