Re: Rejected posting to [e-mail]

Posted by tineke vendrig on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Rejected-posting-to-CONFOCALMICROSCOPY-LISTS-UMN-EDU-tp6866064p6866076.html

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Have you optimised you microscope ?? Kohler illumination and so on..??

Tineke Vendrig, ing
technical engeneer optical microscopy
Delft University of Technology
Bionano Science
Kavli Institute of Nanoscience
Lorentzweg 1
2628LJ Delft
room F185
Tel: [phone]
Fax:[phone]
email: [hidden email]
mobile phone: [phone]


2011/10/6 Hanna Sas Nowosielska <[hidden email]>

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Dear all,
>
> For a couple of months we are struggling with a serious problem with
> the field illumination in our Olympus Scan^R Scanning station. The
> image of the specimen, when observed on the computer screen is much
> darker on one side than on the other. We thought it is the lamp but
> the lamp is new at properly inserted. Then we tried calibrating the
> optic fiber, but it didn't help at all. Our third guess was the
> filters.We checked all of the filters  -  the coating has lots of
> small dark spots with biggest concentration of the spots in the middle
> of the filters, but I am concerned if that can  produce uneven
> illumination of the field so that only one part (let say left corner)
> is underilluminated? So, I turn to you and your experience: did may
> anyone have the similar problem? Do you have any suggestion what can
> be the cause of the problem?
>
> Best regards,
> Hanna
>
> --
> Department of Plant Anatomy and Cytology
> Faculty of Biology and Environmental Protection
> University of Silesia
> Jagiellonska Str. 28
> 40-032 Katowice
> Poland
>

--
Tineke Vendrig, ing
technical engeneer optical microscopy
Delft University of Technology
Bionano Science
Kavli Institute of Nanoscience
Lorentzweg 1
2628LJ Delft
room F185
Tel: [phone]
Fax:[phone]
email: [hidden email]
mobile phone: [phone]