Posted by
Julio Vazquez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Rejected-posting-to-CONFOCALMICROSCOPY-LISTS-UMN-EDU-tp6866064p6866589.html
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When we see similar problems, it tends to be because someone inadvertently bumped a shutter.... start near the light source and work your way along the light path up to the objective, and move any lever or slider you encounter. Our Olympus IX70 has a manual shutter which is a horizontal rotating disk between the filter turret and the objectives. People often bump into the lever and close it partially. On other microscopes, the shutter may be between the lamp and the filter turret. Also, if you have any DIC components, make sure they are either in or out.... sometimes they are half way, and will block part of the illumination field....
--
Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, WA
http://www.fhcrc.org/On Oct 6, 2011, at 7:20 AM, Hanna Sas Nowosielska wrote:
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> Dear all,
>
> For a couple of months we are struggling with a serious problem with
> the field illumination in our Olympus Scan^R Scanning station. The
> image of the specimen, when observed on the computer screen is much
> darker on one side than on the other. We thought it is the lamp but
> the lamp is new at properly inserted. Then we tried calibrating the
> optic fiber, but it didn't help at all. Our third guess was the
> filters.We checked all of the filters - the coating has lots of
> small dark spots with biggest concentration of the spots in the middle
> of the filters, but I am concerned if that can produce uneven
> illumination of the field so that only one part (let say left corner)
> is underilluminated? So, I turn to you and your experience: did may
> anyone have the similar problem? Do you have any suggestion what can
> be the cause of the problem?
>
> Best regards,
> Hanna
>
> --
> Department of Plant Anatomy and Cytology
> Faculty of Biology and Environmental Protection
> University of Silesia
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