Posted by
Cameron Nowell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslips-tp6865241p6867633.html
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Hi Simon,
We use selected Menzel-glaser cover slips that are guaranteed 0.17mm +/-
0.01. Below i have pasted a response to these questions that was on the
list a while ago from Stephen Cody (hope you don't mind Steve).
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalSearch the CONFOCAL archive at
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G'day List,
Probably most of you are already using Menzel coverslips just re-branded
by your suppliers. It's a matter of getting the suppliers to find out
how to order this new sorting of coverslips. In the meantime you can
order them through our supplier here in Australia if you wish. I've just
spoken to them and last time they prepared for an influx of orders
international orders.
I order "0.170 +/- 0.01mm" coverslips for confocal through Lomb
Scientific www.lomb.com.au <
http://www.lomb.com.au/> . This is an
Australian distributor of Menzel Glass. Lomb will also cut coverslips to
special custom sizes (larger bulk orders are more economical).
Peter Bigelow is the fantastic fellow who negotiated on my behalf to
have this grading established.
You can contact Peter directly on
[hidden email] for product
information, order codes etc..
Or as Peter suggests he is not always available
[hidden email] may
work better sometimes.
I only have a catalogue code for the 25mm Round coverslips I order. This
is a Lomb catalogue code rather than a Menzel one.
Code: CSC25(170)GP
Desc: 25mm Coverslip Circle 170 microns (+/- 0.01mm)
Unit: Pack/100
No commercial affiliation with Lomb or Menzel.
Cheers
Stephen H. Cody
Microscopy Manager
Cheers
Cam
Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research Melbourne - Parkville Branch
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
Office: +61 3 9341 3155
Mobile: +61422882700
Fax: +61 3 9341 3104
Facility Website
-----Original Message-----
From: Confocal Microscopy List [mailto:
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On Behalf Of Simon Walker
Sent: Thursday, 6 October 2011 7:43 PM
To:
[hidden email]
Subject: Coverslips
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Hello,
Not strictly a confocal question, but I'm sure someone out there will be
able to
help. We are about to start using a SIM super res system and have been
advised that one of the critical factors in acquiring optimal images is
the cover
glass. Specifcally, the thickness of the glass needs to be consistant
across
the whole coverslip (e.g. 170 um +/- 2 um), and reproducible between
coverslips. One option is to measure each individual coverslip before
use, but
this seems rather impractical to me. Has anyone looked into this, and
if so,
are there any manufacturers out there who can provide cover glass with
this
high specification?
Thanks,
Simon