Re: Coverslips ... 170 +/- 1 um sounds good ... next question: how flat does it need to be for best image quality?

Posted by George McNamara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslips-tp6865241p6867872.html

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Re: Coverslips ... 170 +/- 1 um sounds good.

as for the question of measuring each one: if you are not willing to
take the time and/or spend the money to select optimal coverglasses, you
might as well go back to using a confocal scope.


  ... next question: how flat does it need to be for best image quality?

That is, most slide-specimen-coverglass preparations are hand made - I
do not see how anyone can make them perfectly flat. Has anyone
deliberately (1) perfectly refractive indexed matched everything, and
(2) deliberately tilted the specimen, then quantify image quality? On
the K-inserts, turning the screws on one side might be enough.

thanks in advance,

George


On 10/6/2011 6:41 PM, Cameron Nowell wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Simon,
>
> We use selected Menzel-glaser cover slips that are guaranteed 0.17mm +/-
> 0.01. Below i have pasted a response to these questions that was on the
> list a while ago from Stephen Cody (hope you don't mind Steve).
>
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> G'day List,
>
>
>
> Probably most of you are already using Menzel coverslips just re-branded
> by your suppliers. It's a matter of getting the suppliers to find out
> how to order this new sorting of coverslips. In the meantime you can
> order them through our supplier here in Australia if you wish. I've just
> spoken to them and last time they prepared for an influx of orders
> international orders.
>
>
>
> I order "0.170 +/- 0.01mm" coverslips for confocal through Lomb
> Scientific  www.lomb.com.au<http://www.lomb.com.au/>   . This is an
> Australian distributor of Menzel Glass. Lomb will also cut coverslips to
> special custom sizes (larger bulk orders are more economical).
>
>
>
> Peter Bigelow is the fantastic fellow who negotiated on my behalf to
> have this grading established.
>
> You can contact Peter directly on [hidden email] for product
> information, order codes etc..
>
>
>
> Or as Peter suggests he is not always available [hidden email] may
> work better sometimes.
>
>
>
> I only have a catalogue code for the 25mm Round coverslips I order. This
> is a Lomb catalogue code rather than a Menzel one.
>
>
>
> Code: CSC25(170)GP
>
> Desc: 25mm Coverslip Circle 170 microns (+/- 0.01mm)
>
> Unit:   Pack/100
>
>
>
> No commercial affiliation with Lomb or Menzel.
>
>
>
> Cheers
>
> Stephen H. Cody
> Microscopy Manager
>
>
>
>
>
>
> Cheers
>
> Cam
>
>
> Cameron J. Nowell
> Microscopy Manager
> Centre for Advanced Microscopy
> Ludwig Institute for Cancer Research Melbourne - Parkville Branch
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA
> Office: [phone]
> Mobile: [phone]
> Fax: [phone]
> Facility Website
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Simon Walker
> Sent: Thursday, 6 October 2011 7:43 PM
> To: [hidden email]
> Subject: Coverslips
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello,
> Not strictly a confocal question, but I'm sure someone out there will be
> able to
> help.  We are about to start using a SIM super res system and have been
> advised that one of the critical factors in acquiring optimal images is
> the cover
> glass.  Specifcally, the thickness of the glass needs to be consistant
> across
> the whole coverslip (e.g. 170 um +/- 2 um), and reproducible between
> coverslips.  One option is to measure each individual coverslip before
> use, but
> this seems rather impractical to me.  Has anyone looked into this, and
> if so,
> are there any manufacturers out there who can provide cover glass with
> this
> high specification?
> Thanks,
> Simon
>
>    

--


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami