http://confocal-microscopy-list.275.s1.nabble.com/Coverslips-tp6865241p6867883.html
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> Re: Coverslips ... 170 +/- 1 um sounds good.
>
> as for the question of measuring each one: if you are not willing to take
> the time and/or spend the money to select optimal coverglasses, you might as
> well go back to using a confocal scope.
>
>
> ... next question: how flat does it need to be for best image quality?
>
> That is, most slide-specimen-coverglass preparations are hand made - I do
> not see how anyone can make them perfectly flat. Has anyone deliberately (1)
> perfectly refractive indexed matched everything, and (2) deliberately tilted
> the specimen, then quantify image quality? On the K-inserts, turning the
> screws on one side might be enough.
>
> thanks in advance,
>
> George
>
>
> On 10/6/2011 6:41 PM, Cameron Nowell wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
>> *****
>>
>> Hi Simon,
>>
>> We use selected Menzel-glaser cover slips that are guaranteed 0.17mm +/-
>> 0.01. Below i have pasted a response to these questions that was on the
>> list a while ago from Stephen Cody (hope you don't mind Steve).
>>
>>
>> Search the CONFOCAL archive at
>>
http://listserv.acsu.buffalo.**edu/cgi-bin/wa?S1=confocal<
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
>>
>> Search the CONFOCAL archive at
>>
http://listserv.acsu.buffalo.**edu/cgi-bin/wa?S1=confocal<
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>
>>
>> G'day List,
>>
>>
>>
>> Probably most of you are already using Menzel coverslips just re-branded
>> by your suppliers. It's a matter of getting the suppliers to find out
>> how to order this new sorting of coverslips. In the meantime you can
>> order them through our supplier here in Australia if you wish. I've just
>> spoken to them and last time they prepared for an influx of orders
>> international orders.
>>
>>
>>
>> I order "0.170 +/- 0.01mm" coverslips for confocal through Lomb
>> Scientific www.lomb.com.au<
http://www.**lomb.com.au/<
http://www.lomb.com.au/>>
>> . This is an
>> Australian distributor of Menzel Glass. Lomb will also cut coverslips to
>> special custom sizes (larger bulk orders are more economical).
>>
>>
>>
>> Peter Bigelow is the fantastic fellow who negotiated on my behalf to
>> have this grading established.
>>
>> You can contact Peter directly on
[hidden email] for product
>> information, order codes etc..
>>
>>
>>
>> Or as Peter suggests he is not always available
[hidden email] may
>> work better sometimes.
>>
>>
>>
>> I only have a catalogue code for the 25mm Round coverslips I order. This
>> is a Lomb catalogue code rather than a Menzel one.
>>
>>
>>
>> Code: CSC25(170)GP
>>
>> Desc: 25mm Coverslip Circle 170 microns (+/- 0.01mm)
>>
>> Unit: Pack/100
>>
>>
>>
>> No commercial affiliation with Lomb or Menzel.
>>
>>
>>
>> Cheers
>>
>> Stephen H. Cody
>> Microscopy Manager
>>
>>
>>
>>
>>
>>
>> Cheers
>>
>> Cam
>>
>>
>> Cameron J. Nowell
>> Microscopy Manager
>> Centre for Advanced Microscopy
>> Ludwig Institute for Cancer Research Melbourne - Parkville Branch
>> PO Box 2008
>> Royal Melbourne Hospital
>> Victoria, 3050
>> AUSTRALIA
>> Office: +61 3 9341 3155
>> Mobile: +61422882700
>> Fax: +61 3 9341 3104
>> Facility Website
>>
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:CONFOCALMICROSCOPY@**LISTS.UMN.EDU<
[hidden email]>
>> ]
>> On Behalf Of Simon Walker
>> Sent: Thursday, 6 October 2011 7:43 PM
>> To:
[hidden email].**EDU <
[hidden email]>
>> Subject: Coverslips
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
>> *****
>>
>> Hello,
>> Not strictly a confocal question, but I'm sure someone out there will be
>> able to
>> help. We are about to start using a SIM super res system and have been
>> advised that one of the critical factors in acquiring optimal images is
>> the cover
>> glass. Specifcally, the thickness of the glass needs to be consistant
>> across
>> the whole coverslip (e.g. 170 um +/- 2 um), and reproducible between
>> coverslips. One option is to measure each individual coverslip before
>> use, but
>> this seems rather impractical to me. Has anyone looked into this, and
>> if so,
>> are there any manufacturers out there who can provide cover glass with
>> this
>> high specification?
>> Thanks,
>> Simon
>>
>>
>>
>
>
> --
>
>
> George McNamara, PhD
> Analytical Imaging Core Facility
> University of Miami
>