Posted by
Martin Wessendorf-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslips-tp6865241p6867885.html
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On 10/6/2011 3:43 AM, Simon Walker wrote:
> Not strictly a confocal question, but I'm sure someone out there will be able to
> help. We are about to start using a SIM super res system and have been
> advised that one of the critical factors in acquiring optimal images is the cover
> glass. Specifcally, the thickness of the glass needs to be consistant across
> the whole coverslip (e.g. 170 um +/- 2 um), and reproducible between
> coverslips. One option is to measure each individual coverslip before use, but
> this seems rather impractical to me. Has anyone looked into this, and if so,
> are there any manufacturers out there who can provide cover glass with this
> high specification?
My ignorance is showing here. Why are higher-quality coverslips needed
for SIM? Or is this for live-cell imaging?
Best wishes--
Martin Wessendorf
--
Martin Wessendorf, Ph.D. office: (612) 626-0145
Assoc Prof, Dept Neuroscience lab: (612) 624-2991
University of Minnesota Preferred FAX: (612) 624-8118
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