Quantum yield

Posted by Guy Cox-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Rejected-posting-to-CONFOCALMICROSCOPY-LISTS-UMN-EDU-tp6866064p6867907.html

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I got a rather left field enquiry today, as to whether there were
calibration standards for quantum yield.  It seems that the person wants
to measure quantum yield under the microscope.  My immediate response
was that this is impossible.  Quantum yield is easy enough to measure in
a cuvette but would it be possible in a microscope?  You could make a
standard of a known concentration of fluorescein in a cell made by a
spacer under the coverslip, but where do you go from there, if   both
quantum yield and extinction coefficient of the test sample are unknown?

Any bright ideas?

                                          Guy  


 

Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
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Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
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