Re: Coverslips

Posted by Zac Arrac Atelaz on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslips-tp6865241p6867967.html

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Hello:
 
My guess is that this is required to have more homogeneous and reliable data, maybe you have to ask for the convenience of using sapphire coverslips, this will help you improving your light output.
 
Best Regards
 
Gabriel OH
 

> Date: Thu, 6 Oct 2011 20:26:39 -0500
> From: [hidden email]
> Subject: Re: Coverslips
> To: [hidden email]
>
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> On 10/6/2011 3:43 AM, Simon Walker wrote:
>
> > Not strictly a confocal question, but I'm sure someone out there will be able to
> > help. We are about to start using a SIM super res system and have been
> > advised that one of the critical factors in acquiring optimal images is the cover
> > glass. Specifcally, the thickness of the glass needs to be consistant across
> > the whole coverslip (e.g. 170 um +/- 2 um), and reproducible between
> > coverslips. One option is to measure each individual coverslip before use, but
> > this seems rather impractical to me. Has anyone looked into this, and if so,
> > are there any manufacturers out there who can provide cover glass with this
> > high specification?
>
> My ignorance is showing here. Why are higher-quality coverslips needed
> for SIM? Or is this for live-cell imaging?
>
> Best wishes--
>
> Martin Wessendorf
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