Posted by
Julien Cau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Coverslips-tp6865241p6868429.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
Hi Martin,
You are absolutely right and SIM does not need better coverslips than
any other 0.17 objective-based microscopy technique.
Our users use these coverslips for any experiment (widefield, confocal,
SIM) and they appreciated the difference.
The SIM point is more "don't use a fancy device that promiss you
superesolution if you waste this potential with crap coverslips". If you
buy a wonderfull coffee machine, will you put in it moldy coffee beans?
Wrong thickness coverslips induce decreased light collection, spherical
aberrations and then reduced resolution.
It is worth using them for any type of experiment using a 0.17 lens.
Alternatively, you can buy an expensive lens with a correction collar
that can counteract the effects of the coverslip thickness error.
Best regards
PS : see for instance
http://www.meditec.zeiss.com/4125681F004CA025/Contents-Frame/C66A2521E524C891852575A200721A7C
for a direct comparison of imaging with #1.5 vs #2 coverglass.
Le 07/10/2011 03:26, Martin Wessendorf a écrit :
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> On 10/6/2011 3:43 AM, Simon Walker wrote:
>
>> Not strictly a confocal question, but I'm sure someone out there will
>> be able to
>> help. We are about to start using a SIM super res system and have been
>> advised that one of the critical factors in acquiring optimal images
>> is the cover
>> glass. Specifcally, the thickness of the glass needs to be
>> consistant across
>> the whole coverslip (e.g. 170 um +/- 2 um), and reproducible between
>> coverslips. One option is to measure each individual coverslip
>> before use, but
>> this seems rather impractical to me. Has anyone looked into this,
>> and if so,
>> are there any manufacturers out there who can provide cover glass
>> with this
>> high specification?
>
> My ignorance is showing here. Why are higher-quality coverslips
> needed for SIM? Or is this for live-cell imaging?
>
> Best wishes--
>
> Martin Wessendorf
--
____________________________________________
*/Julien Cau, PhD./*
/Montpellier RIO Imaging Facility manager/Responsable technique MRI/
Montpellier RIO Imaging
Montpellier BIOCAMPUS, UMS3426
Arnaud de Villeneuve Campus Imaging Facility
Institut de Génétique Humaine-CNRS
141, rue de la Cardonille
F-34396 Montpellier(France)
e-mail:
[hidden email].fr_ <mailto:
[hidden email]>
phone: +33.4.34.35.99.90
mobile: +33.6.50.19.27.49
fax: +33.4.34.35.99.01
URL: _http://www.mri.cnrs.fr/_
____________________________________________